CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
| Catalog | IV0102 |
| Product Name | InVivoPro Anti-Mouse CD4 in vivo antibody, Clone GK1.5 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG2b κ |
| Clone | GK1.5 |
| Target | CD4 |
| Other Names | CD4，L3T4，Ly-4 |
| Isotype Control Catalog Code | IV0106 or Rat IgG2b Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 200-500 μg per mouse; or 20 mg/kg. This range is based off the most recent publication data using the GK1.5 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background(ichorbio) | CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation. |
| Applications | In vivo CD4+ T cell depletion, Flow cytometry |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Sade-Feldman, Moshe, et al. "Defining T cell states associated with response to checkpoint immunotherapy in melanoma." Cell 175.4 (2018): 998-1013.
Binnewies, Mikhail, et al. "Unleashing type-2 dendritic cells to drive protective antitumor CD4+ T cell immunity." Cell 177.3 (2019): 556-571.
Yin, **angyun, et al. "Compartmentalized ocular lymphatic system mediates eye–brain immunity." Nature 628.8006 (2024): 204-211.
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