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LPAM-1 is a heterodimer of the 154 kDa integrin α4 (CD49d) and 130 kDa integrin β7 chains and is a member of the Ig superfamily. LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow hematopoietic stem cells.clone DATK32 recognizes mouse lymphocyte Peyer’s patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7 (α4β7).
| Catalog | IV0108 |
| Product Name | InVivoPro Anti-Mouse LPAM-1 (Integrin α4β7) in vivo antibody, Clone DATK32 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG2a κ |
| Clone | DATK32 |
| Target | LPAM-1 |
| Other Names | Integrin α4β7, CD49d/β7, LPAM-1 |
| Isotype Control Catalog Code | IV0105 or Rat IgG2a Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 100-200 μg per mouse. This range is based off the most recent publication data using the DATK32 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background | LPAM-1 is a heterodimer of the 154 kDa integrin α4 (CD49d) and 130 kDa integrin β7 chains and is a member of the Ig superfamily. LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow hematopoietic stem cells.clone DATK32 recognizes mouse lymphocyte Peyer’s patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7 (α4β7). |
| Applications | In vivo Integrin α4β7 neutralization, Flow cytometry, Immunohistochemistry(Frozen) |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
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