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VEGFR-2 is a member of the tyrosine protein kinase family. Upon binding to its ligand VEGF, VEGFR-2 pays key roles in vascular development and permeability. VEGFR-2 is expressed on endothelial cells at high levels in adult heart, lung, kidney, brain, and skeletal muscle as well as other tissues at lower levels. DC101 binds to an extracellular, ligand-binding domain on the amino-terminal of VEGFR-2, thereby blocking VEGFR-2 signaling
| Product Name | InVivoPro Anti-Mouse VEGFR2(CD309) in vivo antibody, Clone DC101 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG1 κ |
| Clone | DC101 |
| Target | VEGFR-2 |
| Other Names | vascular endothelial growth factor receptor 2, CD309, KDR |
| Isotype Control Catalog Code | Rat IgG1 Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 800 μg per mouse; or 40 mg/kg. This range is based off the most recent publication data using the DC101 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background | VEGFR-2 is a member of the tyrosine protein kinase family. Upon binding to its ligand VEGF, VEGFR-2 pays key roles in vascular development and permeability. VEGFR-2 is expressed on endothelial cells at high levels in adult heart, lung, kidney, brain, and skeletal muscle as well as other tissues at lower levels. DC101 binds to an extracellular, ligand-binding domain on the amino-terminal of VEGFR-2, thereby blocking VEGFR-2 signaling |
| Applications | In vivo blocking of VEGFR-2 signaling, Western blot |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Harding, Jeffrey S., et al. "VEGF-A from granuloma macrophages regulates granulomatous inflammation by a non-angiogenic pathway during mycobacterial infection." Cell reports 27.7 (2019): 2119-2131.
Kam, Chen Yuan et al. "Mechanisms of Skin Vascular Maturation and Maintenance Captured by Longitudinal Imaging of Live Mice",Cell 186.11 (2023): 2345-+.
Kabir, Ashraf Ul et al. "Zbtb46 Coordinates Angiogenesis and Immunity to Control Tumor Outcome",NATURE IMMUNOLOGY 25.9 (2024)
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