CD3ε is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
| Catalog | IV0121 |
| Product Name | InVivoPro Anti-Mouse CD3ε in vivo antibody, Clone 145-2C11 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Armenian Hamster IgG |
| Clone | 145-2C11 |
| Target | CD3ε |
| Other Names | T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e |
| Isotype Control Catalog Code | Armenian Hamster IgG Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Armenian Hamster |
| How much antibody to use in vivo | 2-5 μg/mL for in vitro; 200 μg per mouse. This range is based off the most recent publication data using the 145-2C11 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background(ichorbio) | CD3ε is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
| Applications | In vivo T cell depletion, in vitro T cell stimulation, Flow cytometry, Western blot, Immunohistochemistry(Frozen) |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Zanoni, Ivan et al. "IL-15 cis presentation is required for optimal NK cell activation in lipopolysaccharide-mediated inflammatory conditions". Cell reports vol. 4,6 (2013): 1235-49.
Nishikimi, Akihiko et al. "Blockade of inflammatory responses by a small-molecule inhibitor of the Rac activator DOCK2". Chemistry & biology vol. 19,4 (2012): 488-97.
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