Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons. It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops. IFNG has antiviral, immunoregulatory, and anti-tumour properties.
| Catalog | IV0118 |
| Product Name | InVivoPro Anti-Mouse IFNγ in vivo antibody, Clone XMG1.2 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG1 κ |
| Clone | XMG1.2 |
| Target | IFNγ |
| Other Names | Interferon gamma, Ifng |
| Isotype Control Catalog Code | Rat IgG1 Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 200 μg per mouse; or 10 mg/kg. This range is based off the most recent publication data using the XMG1.2 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background(ichorbio) | Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons. It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops. IFNG has antiviral, immunoregulatory, and anti-tumour properties. |
| Applications | In vivo IFNγ neutralization, Western blot, Enzyme-linked Immunosorbent Assay |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Ali, Niwa, et al. "Regulatory T cells in skin facilitate epithelial stem cell differentiation." Cell 169.6 (2017): 1119-1129.
André, Pascale, et al. "Anti-NKG2A mAb is a checkpoint inhibitor that promotes anti-tumor immunity by unleashing both T and NK cells." Cell 175.7 (2018): 1731-1743.
Pasciuto, Emanuela, et al. "Microglia require CD4 T cells to complete the fetal-to-adult transition." Cell 182.3 (2020): 625-640.
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